Rapid breeding method for annual plantlets of dwarfing interstock apple plantlets

专利摘要:
The present invention relates to the technical field of apple cultivation, and particularly relates to a rapid breeding method for annual plantlets of dwarfing interstock apple plantlets. The method 5 is specifically realized by the following steps: (1) preparing rootstock cuttings; (2) preparing dwarfing interstock scions;(3) preparing cultivar scions; (4) grafting; (5) performing sand storage; (6) planting; (7) performing management after planting; and (8) collecting grown plantlets for sales. According to the present invention, the traditional seedlings for rootstocks are replaced with easily rooted stock materials; a root system is provided for apple grafted plantlets; input of 10 means of production such as seeds and production procedures such as seed stratification, seeding and seedlings management are saved. Meanwhile, labour cost is saved by more than 80%; plantlet growing cost is greatly decreased; and labour productivity is greatly improved. The method realizes the synchronization of rootstock rooting process and a growth process of grafted cultivar plantlets, and changes step-by-step grafting of interstock and cultivars to synchronous 15 grafting. The intensive breeding procedure shortens the breeding period of apple plantlets with interstock to one year; and the efficiency is greatly increased.
公开号:NL2025234A
申请号:NL2025234
申请日:2020-03-30
公开日:2020-11-23
发明作者:Wang Haibo；He Ping；Chang Yuansheng；Wang Sen；He Xiaowen；Wang Chuanzeng；Li Linguang；Xu Li
申请人:Shandong Inst Pomology；
IPC主号:

专利说明:

RAPID BREEDING METHOD FOR ANNUAL PLANTLETS OF DWARFING INTERSTOCK
APPLE PLANTLETS Technical Field The present invention relates to the technical field of apple cultivation, and particularly relates to a rapid breeding method for annual plantlets of dwarfing interstock apple plantlets. Background China is a big apple producer in the world, and apple production area and yield exceed 50% of the total quantity in the world. However, a cultivation pattern of vigorous stock dense planting is mainly adopted, and orchards adopting the pattern account for more than 90% of the total area of all orchards. After trees in orchards which adopt the vigorous stock dense planting cultivation manner bear fruits, tree growth size is large, flower bud formation is difficult, and nutrient distribution is unreasonable, so that problems that the total amount of branches in the orchards is relatively large, crowns are close, illumination within the crowns is worse, yield is low, quality is poor, management is difficult, labour amount is large, cost is high and the like are caused, thereby severely restricting the sustainable and healthy development of the apple industry in China.
However, a dwarfing stock intensification pattern is generally adopted in advanced apple production countries in the world, such as Italy, France, America, Australia and New Zealand, and the cultivation area of dwarf stock apple orchards accounts for more than 90% of the total cultivation area of the countries. The orchards that adopt dwarfing stock cultivation have advantages of being early in fruit bearing, fast in efficacy, high in yield, high in quality, high in benefit, convenient in tree management, convenient for mechanized operation, capable of saving labour cost and economically utilizing lands, easy for realization of standardized production, and the like. The dwarfing stock cultivation is a cultivation pattern that is mainly promoted in current apple production in China. By virtue of popularization and application of the dwarfing stock cultivation pattern, transformation and upgrading of the apple industry in China may be effectively pushed, and high-quality sufficient dwarfed stock plantlets are the guarantee of realizing healthy and rapid development of dwarfing cultivation.
At present, plantlets types adopted during establishment of dwarfing stock apple orchards in China are dwarfing interstock apple plantlets or dwarfed self-rooted stock apple plantlets. A Chinese patent (CN201210132808.6) provides a two-year rapid breeding method for high-quality dwarfing interstock apple plantlets. Compared with the conventional method, the method shortens the cultivation time by two years and reduces cost input, but due to the influence of rooting characteristics of apple stock, breeding of the two kinds of plantlets is completed in steps, that is, stock preparation and grafted plantlets cultivation; production links are implemented and completed in fields; and operating conditions are severe and are to be further improved. In the prior art, cultivation of the dwarfing interstock apple plantlets is as follows: rootstock plantlets are bred at first; dwarfing interstock is grafted on the rootstock; then a target cultivar is grafted for cultivation after the dwarfing interstock plantlets are grown up; and the cultivation should be completed within 3 years generally.
However, cultivation of the dwarfing self-rooted stock plantlets is as follows: dwarfing stock plantlets having independent root systems are obtained at first; then the target cultivar is grafted; cultivation of the dwarfing self-rooted stock plantlets is completed by virtue of technologies such as layering, cutting or tissue culture; requirements for the plantlets raising technique and plantlets raising environment are high; operations are complicated; a plantlets raising period lasts for 2-3 years; and the cultivation pattern is difficult to be mastered by a general producer.
Due to the above reasons, problems that breeding operations of the dwarfing stock apple plantlets are complicated, the management period is long, production cost is high and the like are caused, thereby restricting popularization and application of dwarfing cultivation in China.
Summary In order to solve technical problems in an existing dwarfing stock apple plantlets raising technology that an operation technique is complicated, a production period is long, and cost is high, the present invention provides a rapid breeding method for annual plantlets of dwarfing interstock apple plantlets.
According to the method, stock preparation and cultivar grafting can be simultaneously completed on technical operations; plantlets raising steps of the dwarfing stock apple plantlets are greatly simplified; a plantlets raising period is shortened; high-quality plantlets are easily obtained in a short time; production cost is reduced; and plantlets raising efficiency is increased.
Moreover, main technical links of the method may be completed indoors, and an operating environment of the producer is obviously improved.
To solve the above technical problems, the present invention adopts technical solutions as follows: The rapid breeding method for annual plantlets of dwarfing interstock apple plantlets provided by the present invention specifically includes the following steps: (1) preparing rootstock cuttings using an easily rooted stock variety; collecting annual branches from healthy and quarantine pests-free cutting trees in the dormancy period; cutting the collected branches into branch sections of 15-20cm long; burying the branch sections in wet sand; or performing moisturizing treatment and preserving the wet sand in a refrigeration house or a refrigerator at 0-5°C for later use; or using the branches during collection before grafting without preserving; (2) preparing dwarfing interstock scions according to plantlets raising needs, collecting annual branches of a needed dwarfing interstock cultivar in the dormancy period after tree leaves fall; performing branch collection on the healthy and strong stock plants that normally grow and do not have quarantine pests; requiring that the thickness of the collected branches is close to that of the rootstock; cutting the branches into branch sections having a length of 30cm after collection; and burying the branch sections in wet sand for later use; or using the branches during collection before grafting without preserving; (3) preparing cultivar scions according to the plantlets culturing plan, collecting annual branches of a needed grafting cultivar in the dormancy period after leaves fall; performing branch collection on the healthy and strong stock plants that normally grow and do not have quarantine pests; requiring that the thickness of the collected branches is close to that of the interstock; and burying the whole branch section in wet sand, or performing moisturizing treatment, and preserving the wet sand in a refrigeration house or a refrigerator at 0-5°C for later use; or using the branches during collection before grafting without preserving; (4) grafting setting grafting time at 30-45 days before nursery planting of grafted plantlets, that is, early to mid-February every year; grafting interstock branch sections onto rootstock branch sections, and reserving the total length of dwarfing stock branch sections; grafting the cultivar to the upper ends of the interstock branch sections; requiring that the notches are aligned, wrapped and tightened; and simultaneously completing grafting of the interstock and grafting of the cultivar, and forming triple composite plantlets after grafting; (5) performing sand storage immediately performing sand storage preservation after grafting completion, digging a strip- shaped ditch on a leeward and shady spot, covering a layer of clean wet river sand that is 10cm thick and has relative moisture content of 60%, horizontally stacking up the grafted triple composite plantlets on the sand, and requiring that a spacing of 5cm is reserved between every two plantlets; covering a wet sand layer of 10cm thick on a first layer of plantlets after the first layer of plantlets are completely stacked up, and continuously stacking up a second layer of plantlets according to the same manner; covering wet sand on the two layers of plantlets so as to be flush with the ground after the two layers of plantlets are stacked up, and covering earth on the sand until the earth is 10cm higher than the ground; and vertically placing a straw bundle having the diameter of 10cm every 2m in the plantlets stacking and sand covering process, wherein the lower part of the straw bundle extends to the bottom of the ditch, and the upper part of the straw bundle is higher than the ground; (6) planting taking out original plantlets subjected to sand storage in mid-to late-March within 30-45 days after sand storage, and performing planting; and applying base fertilizers to plantlets ridges before planting; (7) performing management after planting immediately watering after planting, and settling soil; hoeing to break the surface soil layer after water on the soil surface evaporates, and preserving soil moisture; inserting an upright bamboo pole beside each plantlets, and enabling the bamboo pole to be close to the plantlets so as to be used for bundling the plantlets; watering in time after planting according to water regime of soil and plantlets appearance, and performing inter tillage weeding; removing buds on the stock in time; performing foliage top-dressing once every 15 days in a vigorous growth period of branches, and continuously performing foliage top-dressing for 3-4 times; and spraying a bactericidal insecticide in combination with the foliage top-dressing for control pests according to pest occurrence conditions; (8) collecting grown plantlets for sales through annual growth and cultivation, enabling the triple composite plantlets planted in spring to grow after leaves fall in autumn, collecting the grown plantlets for sales before soil freezing in early winter of the current year, or collecting the grown plantlets for sales after soil freezing and before plantlets germination in spring of the next year.
Further, in the steps (1) to (3), the relative moisture content of the wet sand is 60%, and the temperature is 0-5°C.
Further, in the step (4), the interstock branch sections are grafted onto the rootstock branch sections by adopting a ‘double tongue grafting’ method.
Further, in the step (4), the cultivar is grafted onto the upper end of the interstock branch sections by adopting a 'double tongue grafting’ method or a wooden bud grafting method.
The triple composite plantlets provided by the present invention are composed of 15-20cm rootstock branch sections, 30cm interstock branch sections and target cultivar branch sections (budding).
Further, in the step (5), depth of the strip-shaped ditch is 40cm, and width of the ditch is 80- 80cm.
Further, in the step (6), depth control is required during planting, and specific control is divided into two manners as follows: 1) for plantlets that can take root on the grafted dwarfing interstock, the rooted rootstock is totally buried into soil during planting, the dwarfing interstock is buried in soil by 10cm, and the rest part is exposed out of the soil; 2) for plantlets that difficultly takes root on the grafted dwarfing interstock, the stock is buried in soil to reach graft union of the rootstock and the interstock during planting, and the rest part is exposed out of the soil.
In the present invention, during planting, planting density is as follows: row spacing is 15- 20cm, and line spacing is 40-60cm.
The rootstock used in the present invention refers to easily rooted stock varieties, such as 'Luzhen #3', 'JM' line, and 'round-leaf Chinese cherry apple’. Branch collection is required to be performed on healthy and strong stock plants that normally grow and do not have quarantine pests in a special cutting orchard or production orchard.
The process of grafting the triple composite plantlets in the present invention may be completed indoors, thereby improving the operating environment and reducing the requirements of the grafting process for the environment.
The present invention has beneficial effects as follows: 5 (1) in the present invention, the traditional seed plantlets are replaced with easily rooted stock resource materials; a root system is provided for apple grafted plantlets; input of means of production such as seeds and production links such as seed stratification, seeding and plantlets management are saved; labour cost is saved by more than 80%;plantlet growing cost is greatly decreased; and labour productivity is improved; (2) a stock rooting process and a growth process of grafted cultivar plantlets are unified; step- by-step grafting of the interstock and the cultivars is modified as synchronous grafting, plantlet growing procedure intensification is realized; a plantlet growing period of dwarfing interstock apple plantlets is shortened from the traditional 2-3 years to 1 year; and plantlet growing efficiency is increased; (3) the stock grafting and cultivar grafting steps in the present invention may be completed indoors and are not influenced by adverse weather; working comfort is increased; and working conditions of producers are improved; (4) when the original plantlets planted in spring end growth in autumn, the height of over 80% of the plantlets may exceed 150cm, the thickness of plantlets above the cultivar graft union is up to 1.0cm, and production needs can be met.
Description of Drawings Fig. 1 is a comparison diagram of rooting capability of 'Luzhen #3' and 'G935' stock; Fig. 2 is a schematic diagram of grafted 'Luzhen #3' / 'SH8' / '2001Fushi' triple composite plantlets; Fig. 3 is a schematic diagram of a 'double tongue grafting' method; Fig. 4 is a comparison diagram of technical processes of a rapid breeding method for annual plantlets of dwarfing interstock apple plantlets in the present invention and a conventional plantlets raising method; Fig. 5 shows an annual rooting situation of rootstock of dwarfing interstock apple plantlets bred by the method in the present invention; and Fig. 6 shows a situation of annual plantlets of dwarfing interstock apple plantlets bred by the method in the present invention.
Detailed Description Technical solutions of the present invention are further explained and described below through specific embodiments.
Embodiment 1 A rapid breeding method for annual plantlets of dwarfing interstock apple plantlets specifically includes the following steps: (1) preparing rootstock cuttings using 'Luzhen #3' stock as a rootstock, wherein the stock is an easily rooted stock variety, as shown in Fig. 1; collecting 'Luzhen #3'annual branches in a tree dormancy period in late January, 2017, wherein the branches are collected from a special cutting orchard in Taolian family farm nursery garden in West Yangzhuang Village, Liancheng Town, Mengyin County, Shandong, and cutting seed trees are healthy and strong in growth and do not have quarantine pests; cutting the collected branches into branch sections of about 20cm long, bundling every 10 branch sections into 1 bundle, burying the branch sections in wet sand having relative moisture content of about 60%, and preserving the wet sand in a refrigeration house at 3+2°Cfor later use; (2) preparing dwarfing interstock scions collecting 'SH6' annual branches of dwarfing interstock as interstock in the tree dormancy period in late January, 2017, wherein the branches are also collected from the special cutting orchard in the Taolian family farm nursery garden in Mengyin County, and cutting seed trees are healthy and strong in growth and do not have quarantine pests; requiring that the thickness of the collected branches is close to that of the rootstock; cutting the collected branches into branch sections of about 30cm long, bundling every 10 branch sections into 1 bundle, burying the branch sections in wet sand having relative moisture content of about 60%, and preserving the wet sand in a refrigeration house at 3£2°Cfor later use; (3) preparing cultivar scions collecting annual branches of a '2001 Fushi’ apple cultivar as scions of a grafted cultivar in the tree dormancy period in late January, 2017, wherein the branches are collected from a demonstration orchard of Shandong Institute of Pomology in Daiyue District, Tai'an City, Shandong, and cutting seed trees are healthy and strong in growth and normal in result and do not have quarantine pests; requiring that the thickness of the collected branches is close to that of the interstock; burying the whole branch sections in wet sand having relative moisture content of about 60% after collection, and preserving the wet sand in a refrigeration house at 3£2°Cfor later use; 4) grafting performing grafting in early February, 2017, wherein the grafting adopts a 'double tongue grafting’ method; grafting interstock'SH6' branch sections onto branch sections of ‘Luzhen #3'rootstock, and reserving the total length of dwarfing stock branch sections; grafting the cultivar '2001 Fushi' to the upper ends of the interstock 'SH86' branch sections by adopting the 'double tongue grafting’ method; requiring that the notches are aligned, wrapped and tightened; and simultaneously completing grafting of the interstock and grafting of the cultivar, and forming triple composite plantlets composed of rootstock branch sections (20cm), interstock branch sections (30cm) and cultivar scion branch sections after grafting, as shown in Fig. 2. The 'double tongue grafting’ method adopted by grafting is as shown in Fig. 3. The operation is performed in the Taolian family farm nursery garden in Mengyin County and completed indoors; (5) performing sand storage immediately performing sand storage preservation after grafting completion, and promoting rooting of rootstock branch sections and healing in graft union; digging a strip-shaped ditch that is 40cm deep and 60-80cm wide on a leeward and shady spot, covering a layer of clean wet river sand that is 10cm thick and has relative moisture content of 60% at the bottom of the ditch, horizontally stacking up the grafted triple composite plantlets on the sand, and requiring that a spacing of 5cm is reserved between every two plantlets; covering a wet sand layer of 10cm thick on a first layer of plantlets after the first layer of plantlets are completely stacked up, and continuously stacking up a second layer of plantlets according to the same manner; covering wet sand on the two layers of plantlets so as to be flush with the ground after the two layers of plantlets are stacked up, and covering earth on the sand until the earth is 10cm higher than the ground, wherein the sand storage depth of the plantlets can be adjusted according to differences of specific areas, and the depth is required to be below a frozen soil layer so as to prevent the plantlets from being frozen; and vertically placing a straw bundle having the diameter of 10cm every 2m in the plantlets stacking and sand covering process, wherein the lower part of the straw bundle extends to the bottom of the ditch, and the upper part of the straw bundle is higher than the ground so as to increase vent ability; the operation is performed in the Taolian family farm nursery garden in Mengyin County and completed indoors; 6) planting taking out original plantlets subjected to sand storage in late March, 2017 within 45 days after sand storage, and performing planting; and applying base fertilizers to plantlets ridges before planting, wherein the base fertilizer adopts rabbit dung that is fully thoroughly decomposed and applied by 2m every 666.7m and a ternary complex fertilizer for apples including 15% of nitrogen, 14% of phosphorus and 16% of potassium is added by 150kg per 666.7m , so as to be fully and uniformly mixed with the surface soil layer of 0-20cm.
The dwarfing interstock in the present embodiment is 'SH8' and belongs to difficultly rooted stock varieties, and during planting, the stock is buried in soil to reach graft union of the rootstock and the interstock, and the rest part is exposed out of the soil; and the planting density is as follows: row spacing is 20cm, and line spacing is 60cm; (7) performing management after planting immediately watering after planting, and settling soil; hoeing to break the surface soil layer after water on the soil surface evaporates, and preserving soil moisture; inserting an upright bamboo pole having the length of about 150cm beside each plantlets, and enabling the bamboo pole to be close to the plantlets so as to be used for bundling the plantlets, thereby ensuring upright growth of the plantlets; watering in time after planting according to water regime of soil and plantlets appearance, and performing inter tillage weeding; removing buds on the stock in time; performing foliage top-dressing once every 20 days from early May, and continuously spraying 0.3% of urea and 0.2% of monopotassium phosphate for 4 times; spraying 50% of thiophanate methyl or 500-time liquid of 50% of carbendazim to control early leaf cast in combination with the foliage top-dressing, and spraying 1500-time liquid of 10% of imidacloprid to control aphides; (8) collecting grown plantlets for sales through annual cultivation, enabling the height of over 80% of the plantlets planted in spring exceeds 150cm when leaves fall in autumn, and enabling the thickness of plantlets above the graft union of interstock/cultivar to be up to 1.2cm, wherein the fibrous root system is developed, and the quantity of fibrous roots is more than 10; and the plantlets are collected for sales before soil freezing in early winter of the current year, thereby completing breeding of the dwarfing interstock apple plantlets.
According to the tripe composite plantlets provided by the present invention, when the rootstock is too short, the fibrous root system is under-developed, and when the rootstock is too long or too short, first-class bred plantlets are decreased compared with the plantlets provided by the present invention.
Compared with the conventional method, the rapid breeding method for annual plantlets of dwarfing interstock apple plantlets in the present invention obtains the results are as follows: (1) The rootstock in the method of the present invention replaces the conventional seed plantlets with the easily rooted stock; the root system is provided for the grown plantlets; links such as stratification, seeding and cultivation in breeding of seed plantlets are saved; step- by-step grafting of the interstock and cultivars is modified as synchronous grafting; and synchronous growth of the rootstock, the interstock and the cultivar is realized by providing favourable fertilizer and water conditions, so that the plantlet growing cost is greatly decreased, and the plantlet growing period is obviously shortened by 2 years. The plantlet growing procedures of the two methods are as shown in Fig. 4 and Fig. 5.
2) When leaves of the dwarfing interstock apple plantlets bred by the method provided by the present invention fall, the height of over 80% of the plantlets exceeds 150cm, the thickness of plantlets above the graft union of interstock/cultivars up to 1.2cm, the fibrous root system is developed, and the quantity of fibrous roots is more than 10, as shown in Fig. 5 and Fig. 6. The standard of the first-class dwarfing interstock apple plantlets specified in Apple Nursery Stocks (GB-9847) is reached.

权利要求:
Claims (8)
[1]
A method for the rapid breeding of annuals from inter-stem apple plants, comprising the steps of: (1) preparing rootstock cuttings using an easily rooted stem variety; collecting annual branches from healthy and quarantined disease-free cutting trees in dormancy; cutting the collected branches into branch pieces with a length of 15 - 20 cm, burying the branch pieces in damp sand; or performing a moistening treatment and storing the moist sand in a cold store or refrigerator at 0 - 5 ° C for later use; or using the branches during collection prior to grafting without preservation; (2) preparing dwarf inter-stem grafts according to the growth needs of the plantlets, collecting annual branches of a required dwarf inter-stem variety in the dormancy period after the tree leaves have fallen; collecting branches on the healthy and strong stock plants that grow normally and do not have quarantine diseases; ensuring that the thickness of the collected branches is close to that of the rootstock; cutting the branches into branch pieces with a length of 30 cm after collecting and burying the branch pieces in moist soil for later use; or using the branches during collection prior to grafting without preservation; (3) preparation of variety grafts according to the plantlet cultivation plan, collecting annual branches of a required grafting variety that is in dormancy after the leaves have fallen; ensuring that the thickness of the collected branches is close to that of the intermediate trunk; and burying the entire branch in damp sand, or performing a moistening treatment and storing the damp sand in a cold store or refrigerator at 0 - 5 ° C for later use; or using the branches during collection prior to grafting without preservation;
(4) inoculating an inoculation period of 30-45 days prior to planting inoculated plantlets in the nursery, i.e. early to mid-February each year; grafting intermediate stem branch pieces onto rootstock branch pieces and reserving the total length of dwarf stem branch pieces; grafting of the variety onto the top ends of the inter-stem branch pieces; ensure the notches are aligned, wrapped and fastened; and simultaneously completing the grafting of the intermediate stem and grafting the variety, and forming tri-composite plantlets after grafting; (5) carry out sand storage immediately after completion of the grafting, keep in sand, dig a strip-shaped trench in a sheltered and shady place, cover with a layer of clean moist river sand 10 cm thick and with a relative humidity of 60%, horizontal raising of the triplicate plantlets on the sand and ensure that a distance of 5 cm is maintained between two plants; cover with a moist sand layer of 10 cm thick on a first layer of plantlets after the first layer of plantlets have been fully raised, and continuously pile up a second layer of plantlets in the same way; cover with moist sand on the two pests of plants to be rinsed with groundwater after the two layers of plants have emerged, and cover with soil on the sand until the soil is 10 c higher than the ground; and vertically placing a reed bundle with a diameter of 10 cm every 2 m in the insertion and sand capping process of the plantlets, with the lower portion of the straw bundle extending to the bottom of the trench, and the upper portion of the straw bundle extending above the ground located; (6) taking out plants from native plantlets that had been subjected to sand storage in mid to late March within 30-45 days of sand storage, and plants; and applying basic fertilizer to plant ridges prior to planting; (7) conducting post-plant management watering immediately after planting and settling the soil; hoeing to break up the surface of the soil layer after the water has evaporated; and retention of soil moisture; inserting an upright bamboo stick next to each plant, and allowing the bamboo sticks to come close to the plants in order to be able to bundle the plants; timely watering according to a watering regime based on the appearance of the plants and soil, weeding the soil between the plants, timely removal of buds on the trunk; top cover of foliage every 15 days during a vigorous growth period of branches, and continue top cover of foliage 3-4 times, and spray with an antibacterial insecticide in combination with top cover of foliage to control diseases according to the conditions of the occurrence of diseases; (8) collecting grown plants for sale by annual growth and growing care of the tri-compound plantlets planted in the spring, after the leaves are galls, collecting the grown plantlets for sale before grounding in the early winter of that year of freezing, or collect the cultivated plants for sale after the ground has frozen and before the plants germinate the following spring.
[2]
The rapid upgrading method according to claim 1, wherein in steps (1) to (3) the relative moisture content of the moist sand is 60% and the temperature is 0-5 ° C.
[3]
The rapid breeding method according to claim 1 or 2, wherein in step (4) the intermediate stem branch pieces are grafted onto the rootstock branch pieces using a "double-tongue" grafting technique.
[4]
The rapid breeding method according to any one of claims 1 to 3, wherein in step (4) the variety is grafted onto the top end of the intermediate stem branches using a 'double-tongued' grafting technique or a wooden bud grafting technique.
[5]
The rapid breeding method according to any of claims 1 to 4, wherein in step (4) the tri-compound plantlets are composed of 15-20 cm rootstock branch pieces, 30 cm intermediate stem branch pieces, and target variety - branch pieces (budding).
[6]
The rapid upgrading method according to any of claims 1 to 5, wherein in step (5) the depth of the strip trench is 40cm and the width of the trench is 60-80cm.
[7]
The rapid breeding method according to any of claims 1 to 6, wherein in step (6) depth control during planting is required and specific control is divided in two ways as follows: (1) for plantlets capable of roots on the grafted dwarf interstock, the rooted rootstock is completely buried in the ground during planting, the dwarf interstock is buried 10 cm in the ground and the remainder is allowed to protrude above the ground; (2) For plantlets having difficulty rooting on the grafted dwarf intermediate stem, the stem is buried in the ground during planting to the graft junction between the rootstock and the intermediate stem and the remainder is allowed to protrude above the ground.
[8]
The rapid breeding method according to claim 7, wherein during planting the density of the plants is as follows: distance between the rows is 15-20 cm, distance between the lines is 40-60 cm.

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CN110115190B|2021-05-04|

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CN110115190A|2019-08-13|

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法律状态:

优先权:

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CN201910371287.1A|CN110115190B|2019-05-06|2019-05-06|Rapid breeding method for apple dwarf interstocks seedling in current year|

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